St-Pierre Lab
Principal Investigator: Francois St-Pierre
Institution: Baylor College of Medicine (Houston, TX)
Primary Subfield: Neuroscience
Recruitment Status: ✅ Hiring Postdocs
Research Focus
PROJECT SUMMARY/ABSTRACT Because neurons integrate and process information via modulation of their membrane potential, the ability to monitor voltage is critical to understanding how single and groups of neurons compute. Genetically encoded voltage indicators (GEVIs) —fluorescent proteins that report voltage dynamics as changes in brightness— are emerging as a preferred recording method because they can track voltage transients with high spatiotemporal resolution and cell type specificity. Particularly sought after are GEVIs that perform well under two-photon (2P) microscopy, the method of choice for imaging neural activity in highly scattering tissue such as the rodent brain. We have recently demonstrated that a combination of the 2P optical recording method ULoVE and the indicator JEDI-2P enable sustained (> 30 min), fast (> 1 kHz), deep-tissue (< 400 µm) monitoring of voltage dynamics in individual neuronal somas in awake behaving mice. However, ULoVE is fundamentally unable to record from cells and structures located in different focal planes. This is a critical limitation as neuronal computations typically involve cells or neurites located at different depths. The goal of this…
Active NIH Awards
- 5R01NS146078-02: Advanced Voltage Indicators Tailored for Two-Photon Resonant Scanning Applications (2026)
- 5U01NS133971-04: ENABLING SUBMILLISECOND-TIMESCALE TWO-PHOTON RECORDING OF VOLTAGE DYNAMICS IN THREE DIMENSIONS IN VIVO (2026)
- 5R01NS136027-03: Red-shifted voltage indicators for two-photon all-optical physiology and multi-spectral imaging (2026)
- 5U01NS133971-03: ENABLING SUBMILLISECOND-TIMESCALE TWO-PHOTON RECORDING OF VOLTAGE DYNAMICS IN THREE DIMENSIONS IN VIVO (2025)
- 5R01EB032854-04: Engineering photostable fluorescent proteins and biosensors using transcriptomic mining and massive-throughput single-cell screening (2025)
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