Iqbal Lab
Principal Investigator: Sumaiya Iqbal
Institution: Broad Institute (Cambridge, MA)
Primary Subfield: Computational Biology
Recruitment Status: Inquire for rotation/fellowship opportunities
Research Focus
PROJECT SUMMARY This proposal aims to develop computational methods for the integrative protein structure-function analysis of base-editor (BE) tiling library and screening results. BE tiling mutagenesis is a high-throughput gene-editing technology to introduce transition variants (A→G and C→T) in cellular models, enabling interrogation of splice-altering, nonsense, and missense mutations. In vitro and in vivo BE experiments are increasingly employed by molecular and chemical biologists to study the mechanisms of mutations and therapeutic targets. However, the scientists face challenges to interpret the experimental readouts, accounting for the molecular effects of these genetic edits, and answer questions such as: “Are the edits unfolding the protein and destabilizing its function?” or “Which edits can recapitulate known drug-resistant mutations or overlap with clinically actionable pathogenic variants?” Currently, answering these questions often requires ad hoc scripting to integrate heterogeneous data types— mRNA-level BE libraries, protein-level mutations, and atomic-resolution structures—and manual queries across disparate databases. These challenges are further amplified when…
Active NIH Awards
- 1R01LM015248-01: Developing Bioinformatics and Computational Biology Methods for Integrative Structure-Function Analysis of CRISPR Base-Editor TilingMutagenesis Experiments (2026)
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