Matreyek Lab
Principal Investigator: Kenneth A Matreyek
Institution: Case Western Reserve University (Cleveland, OH)
Primary Subfield: Synthetic Bio
Recruitment Status: ✅ Hiring Postdocs
Research Focus
PROJECT SUMMARY Advances in high throughput sequencing have already revealed millions of protein coding variants within human exomes, and there are many millions of additional differences that likely exist but have not yet been observed. Many of these variants likely play important roles influencing human health, but we lack the clinical data required to associate each variant genotype with their corresponding phenotypes. This disconnect is oftentimes referred to as the variant interpretation problem. Genetic experiments in model systems play a critical role in uncovering the effects of protein coding variants, but traditional approaches typically test variants one-by-one and will never address this glut of uncharacterized variants. Multiplex genetic assays capable of simultaneously testing complex variant libraries have the required throughput, but these approaches are still in their developmental infancy, and improvements are needed to increase the capabilities, costs, efficiency, and usability of these techniques to successfully address this problem. Harnessing a palette of synthetic biology tools centered around the highly efficient Bxb1 bacteriophage DNA recombinase, I develop…
Active NIH Awards
- 5R35GM142886-05: Recombinant DNA technologies for multiplex genetic assays in human cells (2025)
- 5R35GM142886-04: Recombinant DNA technologies for multiplex genetic assays in human cells (2024)
- 5R21AI178151-02: Large-scale compatibility assessments between ACE2 proteins and diverse sarbecovirus spikes (2024)
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