Prigozhin Lab
Principal Investigator: Maxim Prigozhin
Institution: Harvard University (Cambridge, MA)
Primary Subfield: Neuroscience
Recruitment Status: Inquire for rotation/fellowship opportunities
Research Focus
PROJECT SUMMARY ABSTRACT Ligand-triggered events are central to many processes in neuroscience, endocrinology, virology, immunology, and pharmacology. However, molecular and ultrastructural changes that follow the stimulus are difficult to visualize because they involve rapid nanoscale motions and modifications of proteins and membranes. State-of- the-art techniques are insufficient to capture these spatiotemporal changes. For example, live fluorescence imaging is limited by the spatial resolution (diffraction-limit) and labeling constraints (no antibody access or washing in live cells), while nanoscale imaging methods either lack temporal resolution to capture fast dynamics (e.g., super-resolution optical microscopy) or are incompatible with live-cell imaging altogether (e.g., standard or cryo-electron microscopy; expansion microscopy). Given these limitations, time-resolved cryo-vitrification methods are ideal for capturing cellular processes after a defined wait time post-stimulation by freezing samples in the state of amorphous ice prior to imaging. High-pressure freezing (HPF) is often used for this purpose because of its relaxed sample thickness constraints (<300 µm) as compa…
Active NIH Awards
- 3R01GM146791-05S1: HPF-X: High-pressure freezing with buffer exchange (2026)
- 5R01NS134846-02: Novel Nano- and Immuno-Probes for Multicolor Electron Microscopy of Neural Cells and Tissues (2026)
- 5R01GM146791-05: HPF-X: High-pressure freezing with buffer exchange (2026)
- 5R01GM154030-03: Dynamic nanoscale cellular imaging via time-resolved cryo-vitrification (2026)
- 5R01GM146791-04: HPF-X: High-pressure freezing with buffer exchange (2025)
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