Kural Lab
Principal Investigator: Comert Kural
Institution: The Ohio State University (Columbus, OH)
Primary Subfield: Neuroscience
Recruitment Status: ✅ Hiring Postdocs
Research Focus
PROJECT SUMMARY Neuronal communication depends on the precise coordination of synaptic vesicle exocytosis and endocytosis at presynaptic terminals. After neurotransmitter release, efficient retrieval of synaptic vesicle membranes through endocytosis is essential for sustaining neurotransmission. Despite its fundamental importance, the mechanisms linking synaptic vesicle exocytosis and endocytosis remain poorly understood, largely due to the limitations of current imaging technologies. Existing methods lack the spatial and temporal resolution needed to directly visualize these nanoscale processes in living cells. To address this critical gap, this Joint NINDS/NIMH Exploratory Neuroscience Research Grant (PA-25-150) proposal aims to develop a three-dimensional (3D) total internal reflection fluorescence structured illumination microscope (TIRF-SIM). By integrating super-resolution imaging with evanescent field nanometry, this technology will enable unprecedented visualization of exo-endocytic coupling at the nanoscale in living neurons. Our research plan consists of two key objectives: (i) We will iteratively optimize the experimental parameters of 3D TIRF-SIM using well-characterize…
Active NIH Awards
- 1R21NS143024-01A1: Multi-angle Total Internal Reflection Structured Illumination Microscopy to Investigate Exo-Endocytosis Coupling in Living Cells (2026)
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