Abou Donia Lab
Principal Investigator: Mohamed Abou Donia
Institution: Princeton University (Princeton, NJ)
Primary Subfield: Immunology
Recruitment Status: Inquire for rotation/fellowship opportunities
Research Focus
ABSTRACT A complex interplay exists between the human microbiome and the host, resulting in clear effects on human physiology and microbiome ecology. A promising avenue to dissect this interplay at a mechanistic level is through the study of microbiome-derived molecules that mediate important microbe-microbe and microbe-host interactions. In this application, we propose a hybrid computational- synthetic biology approach to discover, rationally prioritize and systematically characterize microbiome- derived molecules. We propose to apply this approach to three structurally diverse classes of bioactive molecules that are widely encoded by the human microbiome but remain severely understudied in terms of both structure and function. First, guided by the computational analysis of biosynthetic gene clusters in metagenomic sequencing data from the human microbiome of thousands of subjects, we will select specific members of the three molecular classes for experimental characterization. Second, we will use genome editing of native members of the microbiome and synthetic biology in a multi-host heterologous expression platform to characterize the selected pathways and their products. Finall…
Active NIH Awards
- 4R01AI172144-04: Systematic characterization of bioactive molecules from the human microbiome (2025)
- 5R01AI172144-03: Systematic characterization of bioactive molecules from the human microbiome (2024)
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