Jin Lab
Principal Investigator: Xin Jin
Institution: Scripps Research Institute (La Jolla, CA)
Primary Subfield: Genetics/Genomics
Recruitment Status: ✅ Accepting PhD Students ✅ Hiring Postdocs
Research Focus
Human genetic studies have provided lists of genes and loci associated with risk for neuropsychiatric disorders including autism spectrum disorder and neurodevelopmental delay (ASD/ND), but to systematically evaluate their functions and mechanisms remains a dauting task. What brain regions, cell types, and neural circuits are involved in the pathological state? Do different risk genes and genetic variants affect the same sets of brain regions, cell types, neuronal projection networks and circuits? To address these questions, we will develop scalable genomic technologies to allow high-throughput, in vivo genetic screening with single-cell and meso-scale spatial resolution readout from intact tissues. First, we aim establish a method, named Perturb-map, to readout the spatial location, cytoarchitecture, and projectome of each perturbed cell from intact, whole brains without sectioning (Aim 1). We will perform sparse perturbation using the CRISPR-Cas9 system in vivo and harvest the brain with pooled genetic perturbations. Next, we will use hybridization-based detection and wholebrain tissue clearing methods, to allow iterative rounds of light-sheet imaging to deduce gRNA perturbation…
Active NIH Awards
- 5R01HG012819-04: In vivo Perturb-map: scalable genetic screens with single-cell and spatial resolution in intact tissues (2026)
- 5R01MH137042-03: Scalable functional analysis of neuropsychiatric risk genes with spatially integrated in vivo Perturb-seq (2026)
- 5R01HG012819-03: In vivo Perturb-map: scalable genetic screens with single-cell and spatial resolution in intact tissues (2025)
- 5R01MH137042-02: Scalable functional analysis of neuropsychiatric risk genes with spatially integrated in vivo Perturb-seq (2025)
- 5R01HG012819-02: In vivo Perturb-map: scalable genetic screens with single-cell and spatial resolution in intact tissues (2024)
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