Ting Lab
Principal Investigator: Alice Y Ting
Institution: Stanford University (Stanford, CA)
Primary Subfield: Genetics/Genomics
Recruitment Status: Inquire for rotation/fellowship opportunities
Research Focus
Project Summary/Abstract Our research project aims to develop next-generation proximity labeling (PL) enzymes, specifically FlexID and LaccID, to enhance our understanding of molecular interactions and spatial compartmentation within living cells. These principles are foundational to cellular biology; however, current methodologies for mapping cellular interactomes and organelle proteomes, such as microscopy and biochemical fractionation, often lack accuracy and comprehensiveness. Proximity labeling has emerged as a powerful alternative, yet existing methods like APEX and TurboID face significant limitations in specificity, sensitivity, and practicality in vivo. Our proposal seeks to address these challenges. In Aim 1, we will optimize FlexID, an innovative PL enzyme capable of utilizing diverse non-biotin substrates for rapid, non-toxic labeling. This enzyme will be engineered for fast labeling times (within one minute) while maintaining high spatial specificity across various subcellular compartments. Rigorous characterization and improvement of FlexID1 will be conducted through computational design and directed evolution, with the goal of establishing FlexID2 as a versatile tool…
Active NIH Awards
- 1R01GM163165-01: Next-generation proximity labeling enzymes for mapping spatial proteomes in living cells (2026)
- 1R01CA325351-01: Programmable Synthetic GPCRs to Engineer and Understand Cell Behaviors (2026)
- 5R01MH135934-03: Molecular tools for precise manipulation of defined synaptic connections (2026)
- 5R01MH135934-02: Molecular tools for precise manipulation of defined synaptic connections (2025)
- 1R01MH135934-01: Molecular tools for precise manipulation of defined synaptic connections (2024)
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