Ben-Yakar Lab
Principal Investigator: Adela Ben-Yakar
Institution: The University of Texas at Austin (Austin, TX)
Primary Subfield: Molecular & Cell Bio
Recruitment Status: Inquire for rotation/fellowship opportunities
Research Focus
Flow cytometry is the tool of choice for high-speed analysis of large cell populations, with the tradeoff of lacking intracellular spatial information. Imaging flow cytometry (IFC) has emerged as a new tool that combines advantages of microscopy with the high speed of flow cytometry. However, they can only provide 2D images to determine three-dimensional (3D) distribution of cellular features, have a limited field of view (FOV), and require precise control of the fluidic system to minimize image blurring due to uncontrolled cell rotation or translation across the FOV. The absence of 3D imaging results in ambiguity of object locations and blurring by focal depth due to the projection of a 3D cell into a 2D image. Although in the last decades flow cytometry systems that can actually acquire three-dimensional (3D) spatial information were developed, constraints related to resolution and samples size remained as their biggest limitation. Therefore, the goal of this proposal is to develop the next generation 3D imaging flow cytometers with high-throughput and high-content capabilities for 3D imaging of hundreds to thousands of cells and spheroids per second with high resolution, for the…
Active NIH Awards
- 5R01GM148906-04: Three-dimensional fluorescence imaging flow cytometry at up to million frames per second (2026)
- 5R01GM148906-03: Three-dimensional fluorescence imaging flow cytometry at up to million frames per second (2025)
- 5R01GM148906-02: Three-dimensional fluorescence imaging flow cytometry at up to million frames per second (2024)
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