Rothenberg Lab
Principal Investigator: Eli Rothenberg
Institution: New York University (New York, NY)
Primary Subfield: Molecular & Cell Bio
Recruitment Status: Inquire for rotation/fellowship opportunities
Research Focus
PROJECT SUMMARY: PROJECT 4 This project will investigate mammalian DNA polymerase θ (Pol θ), the defining enzyme for repair of DNA double- strand breaks by polymerase theta-mediated end joining (TMEJ). This is Project 4 (“Single-molecule studies of molecular and cellular requirements for Pol θ”), part of a Program Project titled, “Polymerase theta, genome instability, and cancer”. TMEJ is a mutagenic double-stranded break (DSB) repair process vital for cell survival and therapeutic resistance in cancer, particularly those deficient in homologous recombination (HR) repair, such as BRCA-mutated cancers. Despite its significance as a potential cancer therapeutic target, key mechanistic and functional aspects of TMEJ remain poorly understood, hindering the effective application of targeted therapies. This project aims to fill several fundamental gaps in our knowledge of TMEJ, and explore novel hypotheses by employing an array of innovative biochemical, cellular, and single-molecule techniques and assays to define the key steps and molecular mechanisms of TMEJ. First, we will use biochemical reconstitution and single-molecule assays to investigate some of the key interactions and mechan…
Active NIH Awards
- 5P01CA247773-07: Project 4: Single-molecule studies defining molecular and cellular requirements for Pol θ (2026)
- 5R35GM134947-07: Mechanisms of Human DNA Double-Strand Break Repair via Quantitative Single-Molecule Imaging (2026)
- 5P01CA288368-03: Repair Mechanisms of Replication fork Lesions in SCLC (2026)
- 2P01CA247773-06: Project 4: Single-molecule studies defining molecular and cellular requirements for Pol θ (2025)
- 5P01CA288368-02: Repair Mechanisms of Replication fork Lesions in SCLC (2025)
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