Kang Lab
Principal Investigator: Junsu Kang
Institution: University of Wisconsin–Madison (Madison, WI)
Primary Subfield: Molecular & Cell Bio
Recruitment Status: ✅ Accepting PhD Students ✅ Hiring Postdocs
Research Focus
PROJECT SUMMARY/ABSTRACT Zebrafish provide a valuable model system for studying developmental and regenerative biology. Recent advancements in genome editing techniques enable the generation of knock-out alleles in zebrafish, expanding the usefulness of this model organism to interrogate loss-of-function study. However, the majority of genome editing-mediated mutants in zebrafish consist of conventional loss- of-function mutants rather than more sophisticated alleles, such as conditional loss-of-function alleles. Thus, a critical obstacle in zebrafish research lies in the functional dissection of genes within specific cell types and during later development stages. A reliable and definitive conditional loss-of-function system that has been utilized extensively in other organisms is Cre/lox-mediated gene disruption. Although this Cre/lox system is functional in zebrafish, creating reliable floxed alleles has remained challenging. This proposal aims to address the existing limitations in conditional genetics within the zebrafish model by developing a robust and effective methodology. In Aim1, we will establish a synthetic exon-mediated integration approach, allowing the addition of t…
Active NIH Awards
- 5R21OD037634-02: Developing robust genome editing tools for generating floxed alleles and editing amino acid change in zebrafish (2026)
- 5R01HL151522-06: Dissecting gene regulatory networks and regeneration enhancer elements for zebrafish heart regeneration (2026)
- 5R35GM137878-07: Molecular and Genetic Analysis of Fin Regeneration in Zebrafish (2026)
- 1R21OD037634-01A1: Developing robust genome editing tools for generating floxed alleles and editing amino acid change in zebrafish (2025)
- 2R01HL151522-05: Dissecting gene regulatory networks and regeneration enhancer elements for zebrafish heart regeneration (2025)
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