Rinehart Lab
Principal Investigator: Jesse Rinehart
Institution: Yale University (New Haven, CT)
Primary Subfield: Synthetic Bio
Recruitment Status: ✅ Hiring Postdocs
Research Focus
PROJECT SUMMARY Human signaling networks involve large sets of proteins exhibiting highly complex patterns of posttranslational modifications (PTMs) that govern their structure and function. While tremendous progress has been made in identifying PTMs and elucidating the organizational principles of cellular proteins1 (e.g., protein interaction Protein interactions have had a transformative impact on modern medicine. Precision biologics have resulted in breakthroughs in treating diseases (e.g., cancer) and motivate efforts to develop molecular probes that reveal how PTMs could define new targets for targeted drugs. An underlying problem is that protein complexes differentiated by PTMs cannot be separated from non-modified complexes in mammalian cell-based studies. Thus, our knowledge of how PTMs dictate organizational principles of all cellular proteins in human cells is fragmented and few protein domains able to differentiate complex PTM structure have been identified. Here, we aim to leverage advances in protein chemistry and protein engineering to develop an integrated genome and protein engineering high-throughput tool that can differentiate specific PPIs governed by multiple PTMs. Speci…
Active NIH Awards
- 1R01GM163196-01: Development of a synthetic biology tool to engineer proteins with multiple modifications and elucidate their binding function (2026)
- 5R01GM117230-09: Deciphering human signaling networks through synthetic activation of proteins in genomically recoded organisms with multiple open codons (2024)
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